pha eif4e (Addgene inc)
Addgene inc is a verified supplier 
93
Structured Review
Addgene inc
pha eif4e
Pha Eif4e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pha+eif4e/pHA-eIF4E+(Plasmid+%2317343)/pm29112379__co7b00128_si_001-21-64-70
Average 93 stars, based on 20 article reviews
Pha Eif4e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pha+eif4e/pHA-eIF4E+(Plasmid+%2317343)/pm29112379__co7b00128_si_001-21-64-70
Average 93 stars, based on 20 article reviews
pha eif4e - by Bioz Stars,
2026-11
93/100 stars
Images
Related Articles
Clone Assay:Article Title: The Akt-mTOR Pathway Drives Myelin Sheath Growth by Regulating Cap-Dependent Translation Article Snippet: .. The following entry clones were used in this study: p5E: p5E-sox10; p5E-mbpa pME: pME-mNeonCAAX; pME-myrAkt; pME-4EBP1; pME4EBP1T37/46A; pME-mScarletCAAX; pME-eIF4E; pME-mbpaCDS24xMBS; pME-HA-NLS-tdMCP-tagRFP p3E: p3E-P2A-mNeonCAAX; p3E-eGFP; p3E-P2A-myrAkt; p3E-poly A; p3E-mbpa-3’UTR pDEST: pDEST-Tol2-pA pcDNA3-TORCAR (Addgene Plasmid 64927) (Zhou et al., 2015) and pcDNA3-TORCAR(T/A) (Addgene Plasmid 64928) (Zhou et al., 2015) were gifts from Jin Zhang and used to create pME-4EBP1 and pME4EBP1T37/46A, respectively. Article Title: The Akt-mTOR pathway drives myelin sheath growth by regulating cap-dependent translation Article Snippet: .. The following entry clones were used in this study: p5E: p5E-sox10; p5E-mbpa pME: pME-mNeonCAAX; pME-myrAkt; pME-4EBP1; pME-4EBP1 T37/46A ; pME-mScarletCAAX; pME-eIF4E; pME-mbpaCDS-24xMBS; pME-HA-NLS-tdMCP-tagRFP p3E: p3E-P2A-mNeonCAAX; p3E-eGFP; p3E-P2A-myrAkt; p3E-polyA; p3E-mbpa-3’UTR pDEST: pDEST-Tol2-pA pcDNA3-TORCAR (Addgene Plasmid 64927) ( ) and pcDNA3-TORCAR(T/A) (Addgene Plasmid 64928) ( ) were gifts from Jin Zhang and used to create pME-4EBP1 and pME-4EBP1 T37/46A , respectively. Plasmid Preparation:Article Title: The Akt-mTOR Pathway Drives Myelin Sheath Growth by Regulating Cap-Dependent Translation Article Snippet: .. The following entry clones were used in this study: p5E: p5E-sox10; p5E-mbpa pME: pME-mNeonCAAX; pME-myrAkt; pME-4EBP1; pME4EBP1T37/46A; pME-mScarletCAAX; pME-eIF4E; pME-mbpaCDS24xMBS; pME-HA-NLS-tdMCP-tagRFP p3E: p3E-P2A-mNeonCAAX; p3E-eGFP; p3E-P2A-myrAkt; p3E-poly A; p3E-mbpa-3’UTR pDEST: pDEST-Tol2-pA pcDNA3-TORCAR (Addgene Plasmid 64927) (Zhou et al., 2015) and pcDNA3-TORCAR(T/A) (Addgene Plasmid 64928) (Zhou et al., 2015) were gifts from Jin Zhang and used to create pME-4EBP1 and pME4EBP1T37/46A, respectively. Article Title: Cotargeting MNK and MEK kinases induces the regression of NF1 -mutant cancers Article Snippet: .. For Western blots to determine drug efficacy or efficient knockdown, lysates were collected 24 hours following the addition of inhibitors. eIF4E constructs. Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Article Title: The Akt-mTOR pathway drives myelin sheath growth by regulating cap-dependent translation Article Snippet: .. The following entry clones were used in this study: p5E: p5E-sox10; p5E-mbpa pME: pME-mNeonCAAX; pME-myrAkt; pME-4EBP1; pME-4EBP1 T37/46A ; pME-mScarletCAAX; pME-eIF4E; pME-mbpaCDS-24xMBS; pME-HA-NLS-tdMCP-tagRFP p3E: p3E-P2A-mNeonCAAX; p3E-eGFP; p3E-P2A-myrAkt; p3E-polyA; p3E-mbpa-3’UTR pDEST: pDEST-Tol2-pA pcDNA3-TORCAR (Addgene Plasmid 64927) ( ) and pcDNA3-TORCAR(T/A) (Addgene Plasmid 64928) ( ) were gifts from Jin Zhang and used to create pME-4EBP1 and pME-4EBP1 T37/46A , respectively. Western Blot:Article Title: Cotargeting MNK and MEK kinases induces the regression of NF1 -mutant cancers Article Snippet: .. For Western blots to determine drug efficacy or efficient knockdown, lysates were collected 24 hours following the addition of inhibitors. eIF4E constructs. Knockdown:Article Title: Cotargeting MNK and MEK kinases induces the regression of NF1 -mutant cancers Article Snippet: .. For Western blots to determine drug efficacy or efficient knockdown, lysates were collected 24 hours following the addition of inhibitors. eIF4E constructs. Construct:Article Title: Cotargeting MNK and MEK kinases induces the regression of NF1 -mutant cancers Article Snippet: .. For Western blots to determine drug efficacy or efficient knockdown, lysates were collected 24 hours following the addition of inhibitors. eIF4E constructs. Binding Assay:Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Cloning:Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Transfection:Article Title: EIF4E Phosphorylation as a Mechanism of Resistance To mTOR and Androgen Receptor Inhibition in Advanced Prostate Cancer Article Snippet: .. The following plasmids were used in the transfections: pCDNA3 (Invitrogen, Carlsbad, CA), pRL—HCV—FL (kindly provided by Dr. Xinbin Chen, UC Davis School of Veterinary Medicine), pcDNA3—FLAG PTEN (Addgene), Control:Article Title: EIF4E Phosphorylation as a Mechanism of Resistance To mTOR and Androgen Receptor Inhibition in Advanced Prostate Cancer Article Snippet: .. The following plasmids were used in the transfections: pCDNA3 (Invitrogen, Carlsbad, CA), pRL—HCV—FL (kindly provided by Dr. Xinbin Chen, UC Davis School of Veterinary Medicine), pcDNA3—FLAG PTEN (Addgene), other:Article Title: The Androgen Receptor is a negative regulator of eIF4E Phosphorylation at S209: Implications for the use of mTOR inhibitors in advanced prostate cancer Article Snippet: Rapamycin were purchased from LC Laboratories, Massachusetts, USA. |